The 27-year-old singer removed the British comic from the tweeters she follows Friday, reports thesun.co.uk.
But as of Friday night, Brand, whose Twitter handle is "rustyrockets", was still following her.
Brand, 36, filed for divorce Dec 30 citing "irreconcilable differences", after the couple spent Christmas thousands of miles apart.
Perry, who has 14 million Twitter followers, took to the networking site days after the announcement, to tell fans: "You guys have made my heart happy again."
The couple married in India in October 2010. They often exchanged messages on the social networking site.